Janet S. Lee, MD

  • Director, Acute Lung Injury Center of Excellence
  • Director, T1 Translational Research Track, MS program at Institute for Clinical Research Education.
  • Professor, Medicine and Environmental Occupational Health

Education & Training

  • Medical School – Georgetown University – MD
  • Residency – University of Alabama – Internal Medicine
  • Fellowship – University of Washington – Pulmonary/Critical Care Medicine
  • Post-Doctoral Fellowship – University of Washington – Pulmonary/Critical Care Medicine

Research Grants

NIH Grants:  P01, R01, R01, K24

Research Summary

Dr. Lee’s research focuses on the innate arm of immunity, specifically examining the effector function of myeloid cells such as macrophages and neutrophils and how they recognize and respond to exogenous pathogen-associated molecular patterns or endogenous alarmins. Dr. Lee’s laboratory is interested in probing host-pathogen interactions to examine mechanisms of host protection following pathogen-triggered injury from products of extracellular gram-negative pathogens such as Pseudomonas aeruginosa and Klebsiella pneumoniae. Dr. Lee is also interested in the factors that shape repair and resolution following injury, specifically factors derived from hematopoietic cells such as platelets and red blood cells that can influence the course of inflammation.

Representative Publications

  1. Bain W, Olonisakin T, Yu M, Qu Y, Hulver M, Xiong Z, Li H, Pilewski J, Mallampalli R, Ray A, Ray P, Cheng Z, Shanks R, Alexander W, St. Croix C, Silverstein R, Lee JS*.  Platelets inhibit apoptotic lung epithelial cell death and protect mice against infection-induced lung injury.  Blood Adv. 2019 Feb 12;3(3):432-445. PMID: 30733303. PMCID: PMC6373758
  2. Dar HH, Tyurina Y, Mikulska-Raminska K, Shrivastava I, Tyurin V, Krieger J, St. Croix C, Watkins S, Bayir E, Ting HC, Mao G, Ogunsola AF, Flitter R, Freedman C, Gaston JR, Holman T, Pilewski J, Greenberger J, Mallampalli R, Doi Y, Lee JS, Bahar I, Bomberger J, Bayir H, Kagan V. Pseudomonas aeruginosa utilizes host polyunsaturated phosphatidylethanolamines to trigger theft-ferroptosis in bronchial epithelium.  J Clin Invest.  2018 Oct 1;128(10):4639-4653. PMID:30198910. PMCID: PMC6159971
  3. Qu Y, Olonisakin T, Bain W, Zupetic J, Brown R, Hulver M, Xiong Z, Tejero J, Shanks RMQ, Bomberger JM, Cooper VS, Zegans ME, Ryu H, Han J, Pilewski J, Ray A, Cheng Z, Ray P, Lee JS*.  Thrombospondin-1 protects against pathogen-induced lung injury by limiting extracellular matrix proteolysis.  JCI Insight.  2018 Feb 8;3(3). PMID: 29415890; PMCID: PMC5821195
  4. Chakraborty K, Raundhal M, Chen B, Morse C, Tyurina YY, Khare A, Oriss TB, Huff R, Lee JS, St. Croix CM, Watkins S, Mallampalli RK, Kagan V, Ray A, Ray P.  The Mito-DAMP Cardiolipin Blocks IL-10 Production Causing Persistent Inflammation During Bacterial Pneumonia. Nature Communications 2017 Jan 11;8:13944. doi: 10.1038/ncomms13944.  PMID:28074841, PMCID: PMC5241690
  5. Flitter BA, Hvorecny KL, Ono E, Eddens T, Yang J, Kwak DH, Bahl CD, Hampton T, Morisseau C, Hammock BD, Liu X, Lee JS, Kolls JK, Levy BD, Madden DR, Bomberger JM. Pseudomonas aeruginosa sabotages the generation of host pro-resolving lipid mediators.  Proc Natl Acad Sci U S A. 2016 Dec 15. pii: 201610242. [Epub ahead of print].  PMID:  27980032, PMCID: PMC5224368
  6. Olonisakin T, Li HH, Xiong Z, Kochman E.J.K., Yu MT, Qu Y, Hulver M, Kolls JK, St. Croix C, Doi Y, Nguyen MH, Shanks RMQ, Mallampalli RK, Kagan VE, Ray A, Silverstein RL, Ray P, Lee JS*.  CD36 provides host protection against Klebsiella pneumoniae intrapulmonary infection by enhancing LPS responsiveness and macrophage phagocytosis. J Infectious Dis 2016 Sep 28. pii: jiw451. [Epub ahead of print].  PMID:  27683817, PMCID: PMC5142085
  7. Chen K, Eddens T, Trevejo-Nunez G, Way EE, Elsegeiny W, Ricks DM, Garg AV, Erb CJ, Bo M, Wang T, Chen W, Lee JS, Gaffen SL, Kolls JK.  Epithelial IL-17R signaling is required for mucosal chemokine gradients and pulmonary host defense against K. pneumoniae.  Cell Host & Microbe.  2016 Nov 9;20(5):596-605. doi: 10.1016/j.chom.2016.10.003.  PMID: 27923703, PMCID: PMC5149406
  8. Zhao Y, Olonisakin TF, Xiong Z, Hulver M, Sayeed S, Yu MT, Gregory AD, Kochman EJ, Chen BB, Mallampalli RK, Sun M, Silverstein RL, Stolz DB, Shapiro SD, Ray A, Ray P, Lee JS*.  Thrombospondin-1 regulates neutrophil microbial killing by restraining granule serine proteases during K. pneumoniae infection.  Mucosal Immunol. 2015 Jul;8(4):896-905. PMID: 25492474, PMCID: PMC4465063
  9. Zhao Y, Xiong Z, Lechner EJ, Klenotic PA, Hamburg BJ, Hulver M, Khare A, Oriss T, Mangalmurti N, Chan Y, Zhang Y, Ross MA, Stolz DB, Rosengart MR, Pilewski J, Ray P, Ray A, Silverstein RL, Lee JS*.  Thrombospondin-1 triggers macrophage IL-10 production and promotes resolution of experimental lung injury.  Mucosal Immunol. 2014 Mar;7(2):440-8. doi: 10.1038/mi.2013.63. Epub 2013 Sep 18. PMID 24045574, PMCID: PMC3945733
  10. Mangalmurti NS, Xiong Z, Hulver M, Ranganathan M, Liu XH, Oriss T, Fitzpatrick M, Rubin M, Triulzi D, Choi A, Lee JS*.  Loss of Red cell Chemokine Scavenging Promotes Transfusion Related Lung Inflammation.  Blood 2009 Jan 29; 113(5):1158-66.  PMID: 19064726, PMCID: PMC2635081.

Dr. Lee’s NCBI complete listing