Carolyn B. Coyne, PhD

  • Associate Professor, Pediatrics and Microbiology and Molecular Genetics

Education & Training

  • Graduate School – University of North Carolina, Chapel Hill – PhD – Pharmacology
  • Post-Doctoral – University of Pennsylvania - Microbiology

Research Grants

NIH Grants:  R01, R56

Research Summary

The research in my lab combines important aspects of virology, immunology, and cell biology to dissect various aspects of host-virus interactions, with a particular focus on polarized cell types. In particular, we focus on—

  • on the mechanisms by which enteroviruses have evolved to successfully circumnavigate the barriers presented by polarized cells to facilitate virus entry, replication, and spread
  • on the mechanisms utilized by enteroviruses to evade and/or suppress innate immune signaling
  • on the identification of novel regulators and components of the innate immune system
  • on the role of placental-specific microRNAs in antiviral signaling and the induction of autophagy at the maternal-fetal interface.

Representative Publications

  1. Zimmerman MG, Quicke KM, O'Neal JT, Arora N, Machiah D, Priyamvada L, Kauffman RC, Register E, Adekunle O, Swieboda D, Johnson EL, Cordes S, Haddad L, Chakraborty R, Coyne CB, Wrammert J, Suthar MS. Cross-Reactive Dengue Virus Antibodies Augment Zika Virus Infection of Human Placental Macrophages. Cell Host Microbe. 2018 Nov 14;24(5):731-742.e6. doi: 10.1016/j.chom.2018.10.008. PubMed PMID: 30439342.
  2. Freeman MC, Coyne CB, Green M, Williams JV, Silva LA. Emerging arboviruses and implications for pediatric transplantation: A review. Pediatr Transplant. 2018 Oct 19:e13303. doi: 10.1111/petr.13303. [Epub ahead of print] Review. PubMed PMID: 30338634.
  3. Coyne CB. Horsepox: Framing a dual use research of concern debate. PLoS Pathog. 2018 Oct 4;14(10):e1007344. doi: 10.1371/journal.ppat.1007344. eCollection 2018 Oct. PubMed PMID: 30286197; PubMed Central PMCID: PMC6171939
  4. Barrila J, Crabbé A, Yang J, Franco K, Nydam SD, Forsyth RJ, Davis RR, Gangaraju S, Ott CM, Coyne CB, Bissell MJ, Nickerson CA. Modeling Host-Pathogen Interactions in the Context of the Microenvironment: Three-Dimensional Cell Culture Comes of Age. Infect Immun. 2018 Oct 25;86(11). pii: e00282-18. doi: 10.1128/IAI.00282-18. Print 2018 Nov. Review. PubMed PMID: 30181350; PubMed
  5. Coyne CB. STING'ing Zika virus in neurons. Nat Microbiol. 2018 Sep;3(9):975-976. doi: 10.1038/s41564-018-0232-5. PubMed PMID: 30104582.
  6. Lanik WE, Mara MA, Mihi B, Coyne CB, Good M. Stem Cell-Derived Models of Viral Infections in the Gastrointestinal Tract. Viruses. 2018 Mar 10;10(3). pii: E124. doi: 10.3390/v10030124. Review. PubMed PMID: 29534451; PubMed Central PMCID: PMC5869517.
  7. Delorme-Axford E, Abernathy E, Lennemann NJ, Bernard A, Ariosa A, Coyne CB, Kirkegaard K, Klionsky DJ. The exoribonuclease Xrn1 is a post-transcriptional negative regulator of autophagy. Autophagy. 2018;14(5):898-912. doi: 10.1080/15548627.2018.1441648. Epub 2018 Mar 21. PubMed PMID: 29465287; PubMed Central PMCID: PMC6070002.
  8. Ander SE, Rudzki EN, Arora N, Sadovsky Y, Coyne CB, Boyle JP. Human Placental Syncytiotrophoblasts Restrict Toxoplasma gondii Attachment and Replication and Respond to Infection by Producing Immunomodulatory Chemokines. MBio. 2018 Jan 9;9(1). pii: e01678-17. doi: 10.1128/mBio.01678-17. PubMed PMID: 29317509; PubMed Central PMCID: PMC5760739.
  9. Yockey LJ, Jurado KA, Arora N, Millet A, Rakib T, Milano KM, Hastings AK, Fikrig E, Kong Y, Horvath TL, Weatherbee S, Kliman HJ, Coyne CB, Iwasaki A. Type I interferons instigate fetal demise after Zika virus infection. Sci Immunol. 2018 Jan 5;3(19). pii: eaao1680. doi: 10.1126/sciimmunol.aao1680. PubMed PMID: 29305462; PubMed Central PMCID: PMC6049088.
  10. Diamond MS, Coyne CB. Vaccines in 2017: Closing in on a Zika virus vaccine. Nat Rev Immunol. 2018 Feb;18(2):89-90. doi: 10.1038/nri.2017.132. Epub 2017 Dec 4. PubMed PMID: 29199280.
  11. Jagger BW, Miner JJ, Cao B, Arora N, Smith AM, Kovacs A, Mysorekar IU, Coyne CB, Diamond MS. Gestational Stage and IFN-λ Signaling Regulate ZIKV Infection In Utero. Cell Host Microbe. 2017 Sep 13;22(3):366-376.e3. doi: 10.1016/j.chom.2017.08.012. PubMed PMID: 28910635; PubMed Central PMCID: PMC5647680.
  12. Corry J, Arora N, Good CA, Sadovsky Y, Coyne CB. Organotypic models of type III interferon-mediated protection from Zika virus infections at the maternal-fetal interface. Proc Natl Acad Sci U S A. 2017 Aug 29;114(35):9433-9438. doi: 10.1073/pnas.1707513114. Epub 2017 Aug 7. PubMed PMID: 28784796; PubMed Central PMCID: PMC5584447.
  13. Bramley JC, Drummond CG, Lennemann NJ, Good CA, Kim KS, Coyne CB. A Three-Dimensional Cell Culture System To Model RNA Virus Infections at the Blood-Brain Barrier. mSphere. 2017 Jun 21;2(3). pii: e00206-17. doi: 10.1128/mSphere.00206-17. eCollection 2017 May-Jun. PubMed PMID: 28656176; PubMed Central PMCID: PMC5480033.
  14. Arora N, Sadovsky Y, Dermody TS, Coyne CB. Microbial Vertical Transmission during Human Pregnancy. Cell Host Microbe. 2017 May 10;21(5):561-567. doi: 10.1016/j.chom.2017.04.007. Review. PubMed PMID: 28494237; PubMed Central PMCID: PMC6148370.
  15. Dumont TMF, Mouillet JF, Bayer A, Gardner CL, Klimstra WB, Wolf DG, Yagel S, Balmir F, Binstock A, Sanfilippo JS, Coyne CB, Larkin JC, Sadovsky Y. The expression level of C19MC miRNAs in early pregnancy and in response to viral infection. Placenta. 2017 May;53:23-29. doi: 10.1016/j.placenta.2017.03.011. Epub 2017 Mar 16. PubMed PMID: 28487016; PubMed Central PMCID: PMC5506831.
  16. Chang G, Mouillet JF, Mishima T, Chu T, Sadovsky E, Coyne CB, Parks WT, Surti U, Sadovsky Y. Expression and trafficking of placental microRNAs at the feto-maternal interface. FASEB J. 2017 Jul;31(7):2760-2770. doi: 10.1096/fj.201601146R. Epub 2017 Mar 13. PubMed PMID: 28289056; PubMed Central PMCID: PMC5471515.
  17. Drummond CG, Bolock AM, Ma C, Luke CJ, Good M, Coyne CB. Enteroviruses infect human enteroids and induce antiviral signaling in a cell lineage-specific manner. Proc Natl Acad Sci U S A. 2017 Feb 14;114(7):1672-1677. doi: 10.1073/pnas.1617363114. Epub 2017 Jan 30. PubMed PMID: 28137842; PubMed Central PMCID: PMC5320971.